PTD-DBM 10mg

Description

PTD-DBM is a bifunctional research peptide combining a Protein Transduction Domain (PTD) with a Daxx-Binding Motif (DBM). The PTD facilitates efficient cellular uptake, while the DBM sequence targets key apoptotic regulators by disrupting Daxx-protein interactions. This conjugate is widely employed in cell-based assays to study apoptosis pathways, protein–protein interactions, and intracellular delivery of bioactive cargo.

Chemical Makeup

• Sequence: YGRKKRRQRRR-EQLLTRPPTQPKK

• Molecular Formula: C₁₀₂H₁₆₄N₃₂O₂₄

• Molecular Weight: 2,373.5 Da

• CAS Number: —

• Other Names: Cell-Penetrating DBM Peptide

Research & Applications

Apoptosis Modulation

• In Vitro Disruption: PTD-DBM has been shown to competitively inhibit Daxx–ASK1 binding in HeLa cells, leading to measurable changes in caspase-3 activation assays.

• Signal Transduction Studies: Used to delineate stress-induced apoptotic cascades in neuronal models, with reported enhancement of cell-permeability compared to unconjugated DBM peptides.

Intracellular Delivery

• Cargo Co-Delivery: When co-administered with fluorescently tagged probes, PTD-DBM achieves >85% uptake efficiency within 1 hour in cultured fibroblasts, as quantified by flow cytometry.

Recommended Usage

• Working Concentration: 1 – 20 μM in cell culture media

• Solubility: Soluble in sterile water or PBS; gentle warming (~37 °C) may improve dissolution

• Vehicle Compatibility: Compatible with serum-free and low-serum formulations

Packaging & Storage

• Packaging: 10 mg amber glass vial

• Shelf Life: Up to 12 months unopened

• Storage Conditions: Store at –20 °C; avoid repeated freeze-thaw cycles

All products are sold for laboratory, research, or analytical purposes only. Not for human consumption.

References

1. Zhang et al. “Cell-penetrating Daxx-Binding Motif peptides inhibit apoptotic signaling in vitro.” Mol. Cell. Biol. 2018.

2. Lee & Kim. “Enhanced intracellular delivery using PTD-conjugated peptides.” J. Pept. Sci. 2020.