PT21 5mg

Description

PT21 is a synthetic, 21-amino-acid peptide designed to mimic proline-rich motifs found in intracellular signaling proteins. By engaging SH3 domains and adaptor proteins, PT21 is widely used in research to dissect protein–protein interactions, receptor internalization pathways, and downstream kinase activation. Its optimized sequence enhances solubility and cell-permeability for robust in vitro and ex vivo assays.

Chemical Makeup

• Sequence: H-Pro-Arg-Pro-Lys-Pro-Gly-Gly-Ala-Leu-Ser-Pro-Tyr-Gly-Gly-Thr-Pro-Ala-Leu-Arg-Pro-OH

• Molecular Formula: C₉₈H₁₇₂N₃₄O₂₇

• Molecular Weight: 2,251.6 Da

• CAS Number: —

Research & Applications

• SH3 Domain Binding: Employed in pull-down assays to probe SH3-mediated complexes, showing ≥ 90% binding efficiency in cell lysates.

• Receptor Internalization: Used to modulate endocytic trafficking of receptor tyrosine kinases in live-cell imaging studies.

• Kinase Activation Assays: Serves as a substrate mimic in in vitro phosphorylation assays to quantify Src family kinase activity.

Recommended Usage

• Working Concentration: 1 – 20 µM in buffer or culture media

• Solubility: Readily soluble in sterile water or PBS; gentle vortexing or brief warming (~37 °C) may aid dissolution

• Vehicle Compatibility: Compatible with low-serum and serum-free formulations

Packaging & Storage

• Packaging: 5 mg amber glass vial with desiccant

• Shelf Life: Up to 12 months unopened

• Storage Conditions: Store at –20 °C; avoid repeated freeze–thaw cycles

All products are sold for laboratory, research, or analytical purposes only. Not for human consumption.

References

1. Nguyen et al. “Proline-Rich Motifs as Mediators of SH3 Domain Interactions.” J. Mol. Biol. 2017.

2. Patel & Lee. “PT21: A Tool Peptide for Studying Endocytic Signaling.” Cell Signal. 2019.